The neoprene inserts need to be replaced after 1 or 2 uses. If the roots have grown into the neoprene insert & you cannot remove them, you would want to replace the neoprene insert. The decaying roots in the neoprene insert promote rot in the new clone. You must clean cloning systems very thoroughly to make sure that you remove all pathogens with EVERY USE. You should clean your clone system to stop the mister’s from becoming clogged & help stop the pump from becoming clogged. The temperature of the water can also determine clone success rate. You want the water temperature in your clone machine to be 0.